Flow Cytometry Core Facility
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URI: http://hdl.handle.net/2144/2855
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Cloud-based highly parallel execution of t-SNE and SPADE with metaclustering for analysis and visualization of large single-cell datasets
(2017-06)The use of machine learning techniques, in particular unsupervised clustering and dimensionality reduction algorithms, is quickly becoming a standard workflow for identifying and visualizing biological populations from ... -
Automated analysis of 16-color polychromatic flow cytometry data maps immune cell populations and reveals a distinct inhibitory receptor signature in systemic sclerosis
(2015-06)Background. The phenotypic profiles of both peripheral blood and tissue-resident immune cells have been linked to the health status of individuals with infectious and autoimmune diseases, as well as cancer. In light of the ... -
Reagent-driven reconfiguration/optimization of a 16-parameter BD FACSARIA II SORP to allow accurate detection of violet- and UV-excited Sirigen dyes
(2016-06)Background. Modern flow cytometers can detect emission from a variety of commercially available fluorescent reagents. However, accurate detection of novel dyes is often difficult due to the lack of readily available quality ... -
Evaluation of ‘Super Bright’ polymer dyes in 13-16-color human immunophenotyping panels
(2017-06)Sirigen Group Limited developed unique polymer 'Brilliant' dyes that have become a staple of modern multicolor panel design. Polymer-based conjugates are often 4-10 times brighter than conventional fluorochromes with similar ... -
14-color flow cytometry to determine the contribution of mitochondrial mass to differences in glycolytic capacity in human immune cell subsets
(2017-06)Mitochondrial metabolism controls immune cell function, but comprehensive tools to assess human primary immune cell metabolic capacity remain rudimentary. We previously demonstrated that CD19+ B cells rely more heavily on ... -
viSNE fine-tuning enables better resolution of cell populations
(2017-06-09)t-Distributed Stochastic Neighbor Embedding (t-SNE or viSNE) is a dimensionality reduction algorithm that allows visualization of complex high-dimensional cytometry data as a two-dimensional distribution or " map ". These ... -
Role of Fas and Treg cells in fracture healing as characterized in the Fas‐deficient (lpr) mouse model of lupus
(Journal of Bone and Mineral Research, 2014-05-19)Previous studies showed that loss of tumor necrosis factora (TNFa) signaling delayed fracture healing by delaying chondrocyte apoptosis and cartilage resorption. Mechanistic studies showed that TNFa induced Fas expression ... -
Epithelial cell–derived secreted and transmembrane 1a signals to activated neutrophils during pneumococcal pneumonia
(American journal of respiratory cell and molecular biology, 2016-04-11)Airway epithelial cell responses are critical to the outcome of lung infection. In this study, we aimed to identify unique contributions of epithelial cells during lung infection. To differentiate genes induced selectively ... -
Inhibition of Ubc13-mediated ubiquitination by GPS2 regulates multiple stages of B cell development
(The Journal of Biological Chemistry, 2016-12-30)Non-proteolytic ubiquitin signaling mediated by Lys63 ubiquitin chains plays a critical role in multiple pathways that are key to the development and activation of immune cells. Our previous work indicates that GPS2 ... -
Abstract 803: Targeting β-catenin/CBP signaling in OSCC
(AACR Publications, 2017-07)OBJECTIVES: Oral squamous cell carcinoma (OSCC) is an aggressive malignancy characterized by molecular heterogeneity and locoregional spread associated with high morbidity. Aggressive cancers are thought to arise from ...
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